Fig. 1

Flow diagram of the donor selection from the NBB and performed experiments. The selection of donors involved a step by step process aiming to include (depicted in light blue) or exclude (depicted in light red) donors. From the brain collection from the NBB, donors were selected fitting our inclusion criteria. Donors without available frozen tissue of the superior frontal gyrus (SFG), donors with mixed neuropathologies from other disease like multiple sclerosis, but also donors with high amounts of comorbid pathology such as TPD-43 or cortical LBs and donors with psychiatric disorders or symptoms not fitting AD were excluded. Donors with low RIN values (< 6.5) or additional findings of comorbid pathology were replaced. This resulted in 11 resilient donors which were matched with 12 AD patients and 12 control donors. A total of 35 donors were used for the experiments. For immunohistochemistry, donors without any available FFPE tissue were excluded, resulting in 7 resilient, 9 AD and 9 control donors