Fig. 3

Abundant immune infiltration is preserved in psPD events in spite of admixed presentation. (a) Representative 40 × histology of captured regions of interest showing admixed presentation in a sample. (b) Nearest-neighbor distance schematic of segmented nuclei from hematoxylin layer of H&E images among (c) novel enhancement status and (d) status sub-stratified by tissue histology. (e) DAB to hematoxylin staining ratio calculation schematic using image thresholding for Ki67, p53, and Olig2 while CNN-based segmentation was applied for CD163. Staining ratios among (f) novel enhancement status and (g) status sub-stratified by tissue histology. t test: **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Mixed effect modeling was additionally performed with post-hoc p value correction. All comparisons in bolded red represent preserved significance with mixed effect correction