Fig. 6

Modulation of Aβ plaque load following inoculation of Aβ variants. Representative images of 4G8 immunolabeling showing Aβ plaque deposition in the dorsal hippocampus (a-c), subiculum (d-f) and entorhinal cortex (g-i) of APP_swe/PS1_dE9 mice after PBS, Aβ_wt or Aβ_osa inoculation in the dentate gyrus. j-l. Quantification of amyloid load (4G8-positive amyloid plaques per µm²) in the dentate gyrus (j), in the subiculum (k), and in the entorhinal cortex (l). Aβ_osa increases Aβ plaque deposition in the dentate gyrus (p = 0.04), in the subiculum (p = 0.02), in the entorhinal cortex (p = 0.02). Kruskal–Wallis with Dunn’s multiple comparisons. n_APP/PS1-PBS = 10, n_Aβwt = 10, n_Aβosa = 10 mice. Data are shown as mean ± s.e.m. Scale bars main images: 200 µm; Insets: 20 µm