Fig. 1
From: P53 aggregation, interactions with tau, and impaired DNA damage response in Alzheimer’s disease
p53 oligomers and fibrils detected in frontal cortex of human AD brain, not age-matched controls. a Representative confocal images of human AD and age-matched control brain immunofluorescently labeled with anti-p53 (red) and anti-I11 (green; oligomer-specific). b Left Magnified ROI from merged panels in A. Colocalization between p53 and I11 is shown in the AD tissue, but not in control tissue. c Middle: colocalized pixel maps show peri-nuclear overlap of signal with other oligomers and p53 in vicinity. Right: Intensity scatterplot with PCC of 0.86–0.89 indicate a high degree of colocalization suggesting that p53 oligomers occur in AD brain. No colocalized pixel map or PCC calculated for control as there is no detected colocalization. d Fluorescent intensity calculated from AD and control brain for p53 (n = 4 Ctrl; n = 7 AD in technical triplicate) and I11 (n = 4 Ctrl; n = 3 AD in technical triplicate). e Representative confocal images of human AD and age-matched control brain immunofluorescently probed with anti-p53 (red) and anti-OC (green; fibril-specific). f Left Magnified ROI from merged panels in E. Colocalization between p53 and OC is depicted in the AD tissue, but not in control tissue. g Middle: colocalized pixel maps show peri-nuclear overlap of signal with other fibrils and p53 in vicinity. Right: Intensity scatterplot with strong PCC, suggesting that p53 fibrils occur in AD brain. No colocalized pixel map or PCC calculated for control as there is no detected colocalization. h Fluorescent intensity calculated from AD and control brain tissue for OC (n = 4 Ctrl and n = 6 AD in technical triplicate). Keyence Microscope 60X Magnification. Scale bar = 50 μm. i AD and control brain samples heated and then probed by Western blot for p53 (rp53 = recombinant p53 control). j Densitometry analysis of different p53 bands (n = 6 in technical duplicate). k AD and control brain samples heated with reducing agent and then probed by Western blot for p53. l Densitometry analysis of different p53 bands (n = 7 Ctrl; n = 6 AD)