Fig. 3

Significant brain regional difference in formic acid antigen retrieval of LRP. a Low magnification sections from the amygdala and cingulate cortex of an LBD case labeled with antibody 9C10 (residues 2–21 αsyn) without or with FA retrieval as indicated. FA retrieval had a major impact in increasing staining of αsyn aggregates in the amygdala compared with the cingulate cortex. Scale bar 1 mm. b A region of dense Lewy pathology within the amygdala was used to optimize threshold values for positive pixel count analysis. All antibodies were tested to ensure similar detection of pathology with minimal background as shown in bottom panel where red colored pixels are positive. Scale bar 50 μm