Table 5 mEPSC recordings of CT100(I716F)-overexpressing DG granule cells

Frequency [Hz]

0.66 [0.52–0.77]

0.42 [0.3–0.64]

0.89 [0.69–1.63]

1.02 [0.68–1.23]

Kruskal-Wallis: p < 0.0001; Dunn’s posttest: control vs CT100(I716F) p = 0.029; control vs GluN1^−/− p = 0.044; GluN1^−/− vs GluN1^−/− + CT100(I716F) p > 0.99

Percentual reduction

0.36 [0.02–0.55]

−0.15 [− 0.38–0.23]

p = 0.0137

Amplitude [pA]

10.81 [10.03–11.47]

10.62 [10.02–11.13]

11.2 [10.34–12.44]

11.05 [9.78–12]

Kruskal-Wallis: p = 0.3076; Dunn’s posttest: control vs CT100(I716F) p > 0.99; control vs GluN1^−/− p = 0.75; GluN1^−/− vs GluN1^−/− + CT100(I716F) p > 0.99

GluN2A^fl/fl

Control (n= 36)

CT100(I716F) (n= 37)

GluN2A^−/− (n= 24)

GluN2A^−/− + CT100(I716F) (n= 19)

Frequency [Hz]

0.61 [0.5–0.75]

0.5 [0.31–0.68]

0.76 [0.6–0.92]

0.62 [0.46–0.85]

Kruskal-Wallis: p = 0.0004; Dunn’s posttest: control vs CT100(I716F) p = 0.0468; control vs GluN2A^−/− p = 0.149; GluN2A^−/− vs GluN2A^−/− + CT100(I716F) p = 0.485

Percentual reduction

0.19 [− 0.1–0.51]

0.18 [− 0.11–0.39]

p = 0.27

Amplitude [pA]

9.67 [8.46–10.32]

9.83 [9.3–10.82]

10.47 [9.29–12.96]

10.44 [9.84–11.95]

Kruskal-Wallis: p = 0.013; Dunn’s posttest: control vs CT100(I716F) p = 0.844; control vs GluN2A^−/− p = 0.75; GluN2A^−/− vs GluN2A^−/− + CT100(I716F) p > 0.99

GluN2B^fl/fl

Control (n = 28)

CT100(I716F) (n= 25)

GluN2B^−/− (n = 27)

GluN2B^−/− + CT100(I716F) (n = 26)

Frequency [Hz]

0.71 [0.53–1.08]

0.39 [0.28–0.75]

1.01 [0.81–1.23]

0.87 [0.72–1.03]

Kruskal-Wallis: p < 0.0001; Dunn’s posttest: control vs CT100(I716F) p = 0.013; control vs GluN2B^−/− p = 0.018; GluN2B^−/− vs GluN2B^−/− + CT100(I716F) p = 0.497

Percentual reduction

0.45 [− 0.06–0.6]

0.14 [− 0.02–0.29]

p = 0.1

Amplitude [pA]

9.58 [8.61–10.26]

9.65 [8.43–10.51]

9.8 [9.2–10.61]

11.59 [10.16–12.69]

Kruskal-Wallis: p < 0.0001; Dunn’s posttest: control vs CT100(I716F) p > 0.9999; control vs GluN2B^−/− p > .0.9999; GluN2B^−/− vs GluN2B^−/− + CT100(I716F) p = 0.0033