Fig. 3

Specificity of novel tau antibodies raised against tau peptide p193–211 as determined by immunoblotting with recombinant tau proteins phosphorylated in vitro with p42 MAPK or GSK3β. WT, S199A, S202A and T205A 0 N/3R tau (mutants numbered according to 2 N/4R human tau) were incubated with p42 MAPK or GSK3β or without any kinase as described in “Material and Methods.” The proteins were resolved onto 10% polyacrylamide gels and analyzed by immunoblotting with novel tau antibodies (as indicated for each blot) 1H5, 2D1, 3C9, 4A10, 5F2, 6G12, 7F2, 8G5, and 10G12 and total tau antibody H150. The mobilities of molecular mass markers are shown on the left